The use of off-channel anabranch habitat by Murray Cod Maccullochella peelii peelii was studied in the Mullaroo Creek/Lindsay River system, north-western Victoria between 2004 and 2006. Seventy one Murray Cod of varying sizes were captured from the Mullaroo Creek anabranch, Lindsay and Murray rivers. All fish of suitable size were radio-tagged to enable movements to be monitored. Results indicate that the high density of Structural Woody Habitat (SWH) and high velocity habitat found within the Mullaroo Creek system support an exceptional population of Murray Cod compared to nearby Lindsay and Murray River habitats. Most importantly, prior to the breeding season, mature size Murray Cod from the Lindsay and Murray River were recorded to migrate upstream into the upper Mullaroo Creek system where they remained for a considerable period of time before returning to their “home range” habitat downstream. Conversely, individuals with a home range within the upper Mullaroo Creek remained at this site during the spawning period. Spawning site fidelity was clearly recorded over consecutive years for individuals migrating upstream into the upper Mullaroo Creek reach. These results indicate a selection for this habitat within the Lindsay/Chowilla floodplain habitat.