We reviewed the efficiency of the current sampling protocol data for the QLD DPI&F Long Term Monitoring Program with regards to the number of fish species collected. The majority of sites are not exhaustively sampled in any given year but cumulatively almost all of the sites have had 90% or more of their species recorded. The Daintree, Johnstone, Mitchell and Noosa rivers however may have up to 20 species still to be collected. There was very low probability of returning complete species inventories for any of the rivers on any year using the current protocol of seven sites per river. Individual sites within the Condamine were efficiently sampled on any given date and the Warrego river the least likely to have new species in future sampling. If the number of sites were to remain fixed then 9 shots per site would return a more complete species list for most rivers. It would be possible however to adjust the effort at sites independently to avoid over-sampling some sites and under-sampling others. The results are probably influenced by the coastal rivers being under-sampled if they include estuarine sites. Overall, the data have been under-utilised and are a valuable resource that could be put through a suitable bio-monitoring framework for these rivers.