The mean total protein content showed a declination in late embryos resulting from spawning caused by induction using HCG hormone and LHRH hormone each separately, while it showed an increase in the late fertilized embryos resulting from mothers treated with a mixture of HCG + LHRH . On the contrary, the total mean of lipid content showed an opposite results. The total individual amino acid concentrations showed a declination in late embryos resulting from mothers treated with HCG hormone and LHRH hormone each separately showing a normal consumption pattern towards embryos development except Arginine, unlike embryos resulting from mothers treated with a mixture of HCG + LHRH as they showed an increase in the mean total amino acids indicating abnormal consumption pattern except Aspartic acid.

The individual essential and non-essential amino acid profile concentrations of early fertilized embryos showed highly significant differences as P≤0.01 except cystin and isoleucine which showed moderately significant differences as P ≤ 0.05. The essential amino acid Lysine showed the highest concentration level among all acids concerning early fertilized embryos resulting from mothers treated with LHRH hormone, while the non-essential amino acids Proline and Glutamic acid showed the highest concentration level amongst the three treatments in early fertilized embryos resulting from mothers treated using HCG hormone and LHRH hormone each separately.

The qualitative and quantitative determination of fatty acids in the investigated two stages of embryonic development revealed fluctuations among the stages and the hormonal treatment of mothers. Almost all treatments displayed high significant differences in the total concentrations of saturated and unsaturated fatty acids as P ≤0.01. Arachidonic acid is the major precursor, which is involved in the regulation of numerous physiological processes such as growth, development and eicosanoid production. This acid was found in a low concentration in early fertilized embryos resulting from mothers treated with HCG hormone then increased in late embryos of the same treatment indicating better growth of embryos, while in the embryos resulting from other two treatments of mothers it showed a declination in late embryos indicating high consumption and bad formation of membranes and eicosanoid production. The same pattern occurred concerning the concentration of Docosahexanoic acid in early fertilized embryos resulting from mothers treated with HCG hormone, while in late embryos resulting from other two treatments of mothers it showed a declination indicating unsuccessful development of embryos.