The elements contained within calcified structures of fish, molluscs, bivalves and corals may provide a means to determine the environment that an organism has occupied. In order to establish the possible environment of occupancy, it is first important to establish a link between one or more environmental variables and the concentration of elements within its calcified structure. To date, little experimental work has focused on statoliths, especially from cephalopods. Eggs containing prehatchlings, as well as recently hatched giant Australian cuttlefish (Sepia apama) were reared in different concentrations of strontium and barium at three temperatures. The concentrations of Sr:Ca and Ba:Ca in the statoliths were related to water chemistry, but the relationship varied with temperature. Uptake of elements will be compared between prehatchlings and hatchlings, and partition coefficients provided. These results indicate that it is possible to reconstruct the past environmental characteristics from statolith chemistry of cephalopods, based on the concentration of elements in the water. Establishing this link allows for the reconstruction of past environments that cephalopods have occupied based on elemental chemistry.